GCC Shared Core Facilities

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Antibody-Based Proteomics Core, Baylor College of Medicine

The Antibody-based Proteomics Core provides cutting-edge antibody-based proteomic platforms for both validation and protein biomarker discovery, particularly for low-abundance regulatory proteins and activation states of proteins with phosphorylated antibodies.

We currently have an inventory of over 290 validated antibodies that cover multiple total proteins and phosphoproteins in the following protein pathways or functional protein groups: epithelial-mesenchymal transition (EMT), stem cells, apoptosis, DNA damage, autophagy, proliferation and cell cycle, growth factor receptors, cytokines/STATs, nuclear receptors/transcriptional, chromatin regulatory, and metabolism proteins. We continuously work together with BCM investigators to build RPPA assays for new protein pathways of interest and to validate the required antibodies. We are also working to be able to do analysis with various types of samples including cell/tissue lysates, macro-dissected tumor samples, laser capture micro-dissected (LCM) samples, small numbers of isolated stem cells or other rare cell types, and body fluids such as serum and urine. A novel application we have developed is to analyze isolated protein complexes. We have developed an epigenetic RPPA platform to measure histone modifications. We have developed in-house normalization and statistical analyses algorithms.

More information can be found here.

Looking for a particular piece of equipment?

Did you know that the GCC has a Shared Equipment and Resource Committee (SERC) composed of the Directors/Leaders in equipment inventory and acquisition from each of the GCC institutions?  If you are looking for a particular piece of equipment, please email Suzanne Tomlinson, and the committee members will work together to try to locate it and introduce you to the PI who owns/administers it.

GCC Shared Equipment and Cores

Shared core facilities are an important benefit to members of the GCC research community.

GCC has acquired major equipment for shared use, available to Texas Medical Center researchers, thanks to the generosity, commitment and vision of philanthropic donors such as the John S. Dunn Foundation. Additionally, GCC member institutions have each designated shared core facilities within their own institutions (via a GCC inter-institutional memorandum of understanding), providing a large array of equipment available to GCC researchers at internal rates.

These leveraged shared resources enhance collaborative research, training and grant proposals and provide increased opportunities for inter-institutional cooperation and growth. (Photos welcome!) Please contact Suzanne Tomlinson, PhD, Director, GCC Research Consortia, at smtomlin@rice.edu for more information.

Looking for a particular piece of equipment?
Did you know that the GCC has a Shared Equipment and Resource Committee (SERC) composed of the Directors/Leaders in equipment inventory and acquisition from each of the GCC institutions? If you are looking for a particular piece of equipment, please email Suzanne Tomlinson (smtomlin@rice.edu), and the committee members will work together to try to locate it and introduce you to the PI who owns/administers it.

GCC Member Institution Designated Shared Core Facilities